产品名称
PKC α Rabbit Polyclonal Antibody
别名
PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha
蛋白名称
Protein kinase C alpha type
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5578
Human Swissprot No.
P17252
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P17252/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18750
Mouse Swissprot No.
P20444
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P20444
Rat Swissprot Link
http://www.uniprot.org/uniprot/P05696
免疫原
The antiserum was produced against synthesized peptide derived from human PKC alpha. AA range:606-655
特异性
PKC α Polyclonal Antibody detects endogenous levels of PKC α protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2
组织表达
Blood,Brain,Epithelium,Lung,Platelet,
细胞定位
Cytoplasm . Cell membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Nucleus .
信号通路
Regulation_Microtubule; Regulation of Actin Dynamics; Stem cell pathway; Insulin Receptor; ErbB/HER; MAPK_ERK_Growth;MAPK_G_Protein; WNT;WNT-T CELL;β-Catenin; B Cell Receptor; PI3K/Akt; mTOR; AMPK
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domain.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
