产品名称
PIAS 3 Rabbit Polyclonal Antibody
别名
PIAS3; E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3
蛋白名称
E3 SUMO-protein ligase PIAS3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10401
Human Swissprot No.
Q9Y6X2
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y6X2/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=229615
Mouse Swissprot No.
O54714
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54714
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=83614
Rat Swissprot Link
http://www.uniprot.org/uniprot/O70260
免疫原
The antiserum was produced against synthesized peptide derived from human PIAS3. AA range:10-59
特异性
PIAS 3 Polyclonal Antibody detects endogenous levels of PIAS 3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Nucleus . Nucleus speckle . Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules. .
信号通路
Ubiquitin mediated proteolysis;Jak_STAT;Pathways in cancer;Small cell lung cancer;
功能
domain:The LXXLL motif is a transcriptional coregulator signature.,function:Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.,induction:By dihydrotestosterone in prostate cancer cells.,pathway:Protein modification; protein sumoylation.,PTM:Sumoylated.,similarity:Belongs to the PIAS family.,similarity:Contains 1 SAP domain.,similarity:Contains 1 SP-RING-type zinc finger.,subunit:Binds SUMO1 and UBE2I. Interacts with AR, GFI1, HMGA2, IRF1, MITF, NCOA2, as well as with STAT3, after treatment with IL6, CNTF or OSM and with STAT5, after PRL stimulation (By similarity). Interacts with PLAG1.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
.jpg)
