产品名称
PI 3-kinase p101 Rabbit Polyclonal Antibody
别名
PIK3R5; Phosphoinositide 3-kinase regulatory subunit 5; PI3-kinase regulatory subunit 5; PI3-kinase p101 subunit; Phosphatidylinositol 4; 5-bisphosphate 3-kinase regulatory subunit; PtdIns-3-kinase regulatory subunit; Protein FOAP-2; PtdIns-
蛋白名称
Phosphoinositide 3-kinase regulatory subunit 5
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23533
Human Swissprot No.
Q8WYR1
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8WYR1/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=320207
Mouse Swissprot No.
Q5SW28
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q5SW28
免疫原
The antiserum was produced against synthesized peptide derived from human PIK3R5. AA range:695-744
特异性
PI 3-kinase p101 Polyclonal Antibody detects endogenous levels of PI 3-kinase p101 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011],
组织表达
Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.
细胞定位
Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein . Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner. .
信号通路
ErbB_HER;Chemokine;Phosphatidylinositol signaling system;mTOR;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;VEGF;Focal adhesion;Toll_Like;Jak_STAT;Natural killer cell mediated cytotoxicity;T_Cell_Receptor;B_Cell_Antigen;Fc epsilon RI;Fc
功能
domain:The heterodimerization region allows the binding to the catalytic subunit.,enzyme regulation:Greatly activated by G gamma proteins.,function:Regulatory subunit of the PI3K gamma complex.,subunit:Heterodimer of a catalytic subunit (PIK3CG/p120) and a regulatory (PIK3R5a/p101) subunit. Interacts with G beta gamma proteins.,tissue specificity:Highly expressed in leukocytes, followed by spleen, lymph node, thymus ans bone marrow.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
