产品名称
PHLDA3 Rabbit Polyclonal Antibody
别名
PHLDA3; TIH1; Pleckstrin homology-like domain family A member 3; TDAG51/Ipl homolog 1
蛋白名称
Pleckstrin homology-like domain family A member 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=23612
Human Swissprot No.
Q9Y5J5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y5J5/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27280
Mouse Swissprot No.
Q9WV95
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9WV95
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=363989
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q5PQT7
免疫原
The antiserum was produced against synthesized peptide derived from human PHLDA3. AA range:21-70
特异性
PHLDA3 Polyclonal Antibody detects endogenous levels of PHLDA3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
domain:The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.,function:p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.,induction:By p53/TP53; expression is directly activated by p53/TP53. p53/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 promoter.,miscellaneous:PHLDA3 genomic locus is frequently observed in primary lung cancers, suggesting a role in tumor suppression.,similarity:Belongs to the PHLDA3 family.,similarity:Contains 1 PH domain.,tissue specificity:Widely expressed with lowest expression in liver and spleen.,
组织表达
Widely expressed with lowest expression in liver and spleen.
细胞定位
Cytoplasm . Membrane ; Peripheral membrane protein .
功能
domain:The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.,function:p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.,induction:By p53/TP53; expression is directly activated by p53/TP53. p53/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 promoter.,miscellaneous:PHLDA3 genomic locus is frequently observed in primary lung cancers, suggesting a role in tumor suppression.,similarity:Belongs to the PHLDA3 family.,similarity:Contains 1 PH domain.,tissue specificity:Widely expressed with lowest expression in liver and spleen.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
