产品名称
PHF1 Rabbit Polyclonal Antibody
别名
PHF1; PCL1; PHD finger protein 1; Protein PHF1; Polycomb-like protein 1; hPCl1
蛋白名称
PHD finger protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5252
Human Swissprot No.
O43189
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43189/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21652
Mouse Swissprot No.
Q9Z1B8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9Z1B8
免疫原
The antiserum was produced against synthesized peptide derived from human PHF1. AA range:345-394
特异性
PHF1 Polyclonal Antibody detects endogenous levels of PHF1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
组织表达
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
细胞定位
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Co-localizes with NEK6 in the centrosome.
功能
function:Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.,similarity:Contains 2 PHD-type zinc fingers.,subcellular location:Localizes specifically to the promoters of numerous target genes.,subunit:Interacts with CHMP1 (By similarity). Interacts with the PRC2 complex.,tissue specificity:Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
