产品名称
Periphilin 1 Rabbit Polyclonal Antibody
别名
PPHLN1; HSPC206; HSPC232; Periphilin-1; Gastric cancer antigen Ga50
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=51535
Human Swissprot No.
Q8NEY8
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8NEY8/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=223828
Mouse Swissprot No.
Q8K2H1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K2H1
免疫原
The antiserum was produced against synthesized peptide derived from human PPHLN. AA range:71-120
特异性
Periphilin 1 Polyclonal Antibody detects endogenous levels of Periphilin 1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
细胞定位
Nucleus . Cytoplasm . Chromosome . In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions (PubMed:12853457). Localizes to chromatin (PubMed:26022416). .
功能
function:Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.,PTM:Substrate of transglutaminase (in vitro).,subcellular location:In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.,subunit:Homodimer. Interacts with PPL.,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
