产品名称
PEDF Rabbit Polyclonal Antibody
别名
SERPINF1; PEDF; PIG35; Pigment epithelium-derived factor; PEDF; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1
蛋白名称
Pigment epithelium-derived factor
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5176
Human Swissprot No.
P36955
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P36955/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20317
Mouse Swissprot No.
P97298
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P97298
免疫原
The antiserum was produced against synthesized peptide derived from human PEDF. AA range:258-307
特异性
PEDF Polyclonal Antibody detects endogenous levels of PEDF protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016],
组织表达
Retinal pigment epithelial cells and blood plasma.
细胞定位
Secreted . Melanosome . Enriched in stage I melanosomes.
功能
developmental stage:Expressed in quiescent cells.,domain:The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.,function:Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.,PTM:The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.,similarity:Belongs to the serpin family.,subcellular location:Enriched in stage I melanosomes.,tissue specificity:Retinal pigment epithelial cells and blood plasma.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
