产品名称
PDZ-RhoGEF Rabbit Polyclonal Antibody
别名
ARHGEF11; KIAA0380; Rho guanine nucleotide exchange factor 11; PDZ-RhoGEF
蛋白名称
Rho guanine nucleotide exchange factor 11
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9826
Human Swissprot No.
O15085
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O15085/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=78966
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9ES67
免疫原
The antiserum was produced against synthesized peptide derived from human ARHGEF11. AA range:198-247
特异性
PDZ-RhoGEF Polyclonal Antibody detects endogenous levels of PDZ-RhoGEF protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitously expressed.
细胞定位
Cytoplasm . Membrane . Translocated to the membrane upon stimulation.
信号通路
Regulation of Actin Dynamics; AMPK
功能
domain:The poly-Pro region is essential for plasma membrane localization upon stimulation.,function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13 through the RGS domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts with SLC1A6.,tissue specificity:Ubiquitously expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
