BD-PT3645-PDK1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PDK1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT3645

产品名称

PDK1 Rabbit Polyclonal Antibody

别名

PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1

类别

常规抗体

基因名称

PDPK1

蛋白名称

3-phosphoinositide-dependent protein kinase 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5170

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5170

Human Swissprot No.

O15530

Human Swissprot Link

http://www.uniprot.org/uniprotkb/O15530/entry

Mouse Gene ID

18607

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18607

Mouse Swissprot No.

Q9Z2A0

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9Z2A0

Rat Gene ID

81745

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81745

Rat Swissprot No.

O55173

Rat Swissprot Link

http://www.uniprot.org/uniprot/O55173

免疫原

The antiserum was produced against synthesized peptide derived from human PDK1. AA range:210-259

特异性

PDK1 Polyclonal Antibody detects endogenous levels of PDK1 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

60kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,

组织表达

Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.

细胞定位

Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizes with PTK2B in focal adhesions after angiotensin II stimulation.

信号通路

PPAR;mTOR;Focal adhesion;Insulin_Receptor;Aldosterone-regulated sodium reabsorption;Endometrial cancer;Prostate cancer;Non-small cell lung cancer;

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PDK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from MDA-MB-435 cells, treated with EGF, using PDK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-heart tissue. 1,PDK1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,PDK1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,PDK1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using PDK1 Polyclonal Antibody diluted at 1：1000

Western Blot analysis of COLO205 cells using PDK1 Polyclonal Antibody diluted at 1：1000