BD-PT3617

PCNA Rabbit Polyclonal Antibody

PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

常规抗体

PCNA

Proliferating cell nuclear antigen

WB

Human,Mouse,Rat,Monkey,Rabbit

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

5111

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5111

P12004

http://www.uniprot.org/uniprotkb/P12004/entry

18538

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18538

P17918

http://www.uniprot.org/uniprot/P17918

25737

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25737

P04961

http://www.uniprot.org/uniprot/P04961

The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110

PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.

WB 1:500-2000;IHC-p 1:100-500;IF ICC 1:100-500;ELISA 1:5000-20000

30-33kd

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

Bone marrow,Fetal brain cortex,Lung,Placenta,

Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .

Protein_Acetylation

disease:Antibodies are present in sera from patients with systemic lupus erythematosus.,function:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.,online information:PCNA entry,PTM:Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases RNF8 and SHPRH, which are required for DNA repair.,similarity:Belongs to the PCNA family.,subunit:Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH. Forms a ternary complex with DNTTIP2 and core histone. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B/WSTF; the interaction is direct.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.