产品名称
PCAF Rabbit Polyclonal Antibody
别名
KAT2B; PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Histone acetylase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; P/CAF
蛋白名称
Histone acetyltransferase KAT2B
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8850
Human Swissprot No.
Q92831
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q92831/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18519
Mouse Swissprot No.
Q9JHD1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JHD1
免疫原
The antiserum was produced against synthesized peptide derived from human p300/CBP. AA range:783-832
特异性
PCAF Polyclonal Antibody detects endogenous levels of PCAF protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitously expressed but most abundant in heart and skeletal muscle. Also expressed in the skin, in keratinocytes (at protein level) (PubMed:20940255).
细胞定位
Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm . Mainly localizes to the nucleus. Also localizes to centrosomes in late G1 and around the G1/S transition, coinciding with the onset of centriole formation. Subcellular location may vary depending upon cell differentiation state. Cytoplasmic at the very stages of keratinocyte differentiation, becomes nuclear at later differentiation stages. Cytoplasmic in basal epithelial cells (undifferentiated cells) and nuclear in parabasal cells (differentiated cells) (PubMed:20940255). .
功能
chromatin organization,?chromatin remodeling,?transcription,?regulation of transcription, DNA-dependent,?protein amino acid acetylation,?N-terminal protein amino acid acetylation,?cell cycle,?cell cycle arrest,?negative regulation of cell proliferation,?response to endogenous stimulus,?response to hormone stimulus,?response to organic substance,chromatin modification,?covalent chromatin modification,?histone modification,?histone acetylation,?N-terminal peptidyl-lysine acetylation,?peptidyl-lysine modification,?peptidyl-lysine acetylation,?cell cycle process,?N-terminal protein amino acid modification,?response to insulin stimulus,?cellular response to insulin stimulus,?cellular response to hormone stimulus,?regulation of cell proliferation,?response to peptide hormone stimulus,?protein amino acid acylation,regulation of transcription,?regulation of RNA metabolic process,?chromosome organization,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
