产品名称
PBK Rabbit Polyclonal Antibody
别名
PBK; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinas
蛋白名称
Lymphokine-activated killer T-cell-originated protein kinase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55872
Human Swissprot No.
Q96KB5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q96KB5/entry
Mouse Swissprot No.
Q9JJ78
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JJ78
免疫原
The antiserum was produced against synthesized peptide derived from human PBK/TOPK. AA range:1-50
特异性
PBK Polyclonal Antibody detects endogenous levels of PBK protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
PDZ binding kinase(PBK) Homo sapiens This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
组织表达
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by phosphorylation.,function:Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.,PTM:Phosphorylated; in a cell-cycle dependent manner at mitosis.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with DLG1 and TP53.,tissue specificity:Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
