产品名称
Paxillin Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5829
Human Swissprot No.
P49023
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49023/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19303
Mouse Swissprot No.
Q8VI36
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8VI36
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=360820
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q66H76
免疫原
The antiserum was produced against synthesized peptide derived from human Paxillin. AA range:144-193
特异性
Paxillin Polyclonal Antibody detects endogenous levels of Paxillin protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011],
组织表达
Brain,Epithelium,Lung,Placenta,T-cell,Uterus,
细胞定位
Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389). .
信号通路
Chemokine;VEGF;Focal adhesion;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;
功能
function:Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).,PTM:Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.,similarity:Belongs to the paxillin family.,similarity:Contains 3 LIM zinc-binding domains.,similarity:Contains 4 LIM zinc-binding domains.,subunit:Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
