BD-PT3497-p21 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

p21 Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT3497

产品名称

p21 Rabbit Polyclonal Antibody

别名

CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21

类别

常规抗体

基因名称

CDKN1A

蛋白名称

Cyclin-dependent kinase inhibitor 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1026

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1026

Human Swissprot No.

P38936

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P38936/entry

Mouse Gene ID

12575

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12575

Mouse Swissprot No.

P39689

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P39689

免疫原

The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160

特异性

p21 Polyclonal Antibody detects endogenous levels of p21 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

21kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lac

组织表达

Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

细胞定位

Cytoplasm . Nucleus .

信号通路

Stem cell pathway; ErbB/HER; PI3K/Akt; AMPK; Cell_Cycle_G1S;Cell_Cycle_G2M_DNA; Protein_Acetylation

功能

function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from COLO and K562 cells, treated with EGF, using p21 Cip1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of mouse-kidney tissue. 1,p21 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,p21 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,p21 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,p21 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,p21 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using p21 Polyclonal Antibody

Western Blot analysis of COLO205 cells using p21 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human Right liver. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.