BD-PT3492-p15 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

p15 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT3492

产品名称

p15 Rabbit Polyclonal Antibody

别名

CDKN2B; MTS2; Cyclin-dependent kinase 4 inhibitor B; Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B

类别

常规抗体

基因名称

CDKN2B

蛋白名称

Cyclin-dependent kinase 4 inhibitor B

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1030

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1030

Human Swissprot No.

P42772

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P42772/entry

Mouse Gene ID

12579

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12579

Mouse Swissprot No.

P55271

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P55271

Rat Gene ID

25164

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25164

Rat Swissprot No.

P55272

Rat Swissprot Link

http://www.uniprot.org/uniprot/P55272

免疫原

The antiserum was produced against synthesized peptide derived from human p15 INK. AA range:89-138

特异性

p15 Polyclonal Antibody detects endogenous levels of p15 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

14kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008],

组织表达

Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.

细胞定位

Cytoplasm . Also found in the nucleus.

信号通路

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;TGF-beta;Pathways in cancer;Small cell lung cancer;

功能

disease:Defects in CDKN2B are involved in tumor formation.,function:Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.,polymorphism:Genetic variations in CDKN2B may underlie susceptibility to uveal melanoma [MIM:155720]. Uveal melanoma is the most common type of ocular malignant tumor, consisting of overgrowth of uveal melanocytes and often preceded by a uveal nevus.,similarity:Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.,similarity:Contains 4 ANK repeats.,subunit:Heterodimer of CDKN2B with CDK4 or CDK6.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using p15 INK Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p15 INK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using p15 INK Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using p15 Polyclonal Antibody diluted at 1：500

Immunohistochemical analysis of paraffin-embedded Human Right liver. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.