产品名称
p130 Cas Rabbit Polyclonal Antibody
别名
BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
蛋白名称
Breast cancer anti-estrogen resistance protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9564
Human Swissprot No.
P56945
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P56945/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12927
Mouse Swissprot No.
Q61140
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61140
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25414
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63767
免疫原
The antiserum was produced against synthesized peptide derived from human p130 Cas. AA range:131-180
特异性
p130 Cas Polyclonal Antibody detects endogenous levels of p130 Cas protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009],
组织表达
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
细胞定位
Cell junction, focal adhesion . Cytoplasm . Cell projection, axon . Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity). .
信号通路
Chemokine;Focal adhesion;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;
功能
domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix.,similarity:Belongs to the CAS family.,similarity:Contains 1 SH3 domain.,subcellular location:Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.,subunit:Forms complexes in vivo with focal adhesion kinase 1, adapter protein CRKL and LYN kinase. Can heterodimerize with CASL. Interacts with BCAR3, NPHP1, PTK2B and SH2D3C (By similarity). Interacts with activated CSPG4. Interacts with INPPL1/SHIP2.,tissue specificity:Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
