BD-PT3230-Oct-1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Oct-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT3230

产品名称

Oct-1 Rabbit Polyclonal Antibody

别名

POU2F1; OCT1; OTF1; POU domain; class 2, transcription factor 1; NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1

类别

常规抗体

基因名称

POU2F1

蛋白名称

POU domain class 2 transcription factor 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5451

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5451

Human Swissprot No.

P14859

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P14859/entry

Mouse Gene ID

18986

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18986

Mouse Swissprot No.

P25425

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P25425

免疫原

The antiserum was produced against synthesized peptide derived from human OCT1. AA range:41-90

特异性

Oct-1 Polyclonal Antibody detects endogenous levels of 40817 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

预测分子量

76kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010],

组织表达

Ubiquitous. Isoform 2 is lymphocyte-specific.

细胞定位

Nucleus.

功能

function:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.,PTM:Phosphorylated by PRKDC.,similarity:Belongs to the POU transcription factor family. Class-2 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 POU-specific domain.,subunit:Interacts with NR3C1, AR, PGR and HCFC1.,tissue specificity:Ubiquitous. Isoform 2 is lymphocyte-specific.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, using OCT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using OCT1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Oct-1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of HeLa cells using Oct-1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.