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NY-CO-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT3215
产品名称
NY-CO-1 Rabbit Polyclonal Antibody
别名
NEMF; SDCCAG1; Nuclear export mediator factor NEMF; Antigen NY-CO-1; Serologically defined colon cancer antigen 1
类别
常规抗体
基因名称
NEMF
蛋白名称
Nuclear export mediator factor NEMF
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
9147
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9147
Human Swissprot No.
O60524
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O60524/entry
Mouse Gene ID
66244
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=66244
Mouse Swissprot No.
Q8CCP0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8CCP0
免疫原
The antiserum was produced against synthesized peptide derived from human SDCG1. AA range:881-930
特异性
NY-CO-1 Polyclonal Antibody detects endogenous levels of NY-CO-1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
预测分子量
123kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016],
组织表达
Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis.
细胞定位
Cytoplasm, cytosol . Nucleus .
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using NY-CO-1 Polyclonal Antibody

Western Blot analysis of 3T3 cells using NY-CO-1 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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