产品名称
NXF3 Rabbit Polyclonal Antibody
别名
NXF3; TAPL3; Nuclear RNA export factor 3; TAP-like protein 3; TAPL-3
蛋白名称
Nuclear RNA export factor 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56000
Human Swissprot No.
Q9H4D5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9H4D5/entry
免疫原
The antiserum was produced against synthesized peptide derived from human NXF3. AA range:72-121
特异性
NXF3 Polyclonal Antibody detects endogenous levels of NXF3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq, Jul 2008],
组织表达
Expressed at high level in testis and at low level in a small number of tissues.
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
功能
domain:Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain.,domain:Lacks C-terminal domain that mediates direct interactions with nucleoporins.,function:May function as a tissue-specific nuclear mRNA export factor.,miscellaneous:The RNA-binding domain is a non-canonical RNP-type domain.,similarity:Belongs to the NXF family.,similarity:Contains 1 LRR (leucine-rich) repeat.,similarity:Contains 1 NTF2 domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Shuttles between the nucleus and the cytoplasm.,subunit:Interacts with NXT1, NXT2, E1B-AP5 and CRM1 nuclear export factor.,tissue specificity:Expressed at high level in testis and at low level in a small number of tissues.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
