产品名称
NUB1 Rabbit Polyclonal Antibody
别名
NUB1; NYREN18; NEDD8 ultimate buster 1; Negative regulator of ubiquitin-like proteins 1; Renal carcinoma antigen NY-REN-18
蛋白名称
NEDD8 ultimate buster 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=51667
Human Swissprot No.
Q9Y5A7
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y5A7/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=53312
Mouse Swissprot No.
P54729
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P54729
免疫原
The antiserum was produced against synthesized peptide derived from human NYREN18. AA range:566-615
特异性
NUB1 Polyclonal Antibody detects endogenous levels of NUB1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splici
组织表达
Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
细胞定位
Nucleus . Predominantly nuclear.
功能
function:Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8 and its conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.,induction:By beta and gamma interferons.,similarity:Contains 3 UBA domains.,subcellular location:Predominantly nuclear.,subunit:Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Isoform 1 binds to NEDD8 more efficiently than isoform 2. Interacts with AIPL1.,tissue specificity:Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
