BD-PK0003-Histone H2B (Acetyl Lys5) Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Histone H2B (Acetyl Lys5) Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PK0003

产品名称

Histone H2B (Acetyl Lys5) Rabbit Polyclonal Antibody

别名

H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s;H2BK5AC

类别

常规抗体

基因名称

H2BFS

蛋白名称

Histone H2B type F-S

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

54145

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54145

Human Swissprot No.

P57053

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P57053/entry

免疫原

The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys5. AA range:1-50

特异性

Acetyl-Histone H2B (K5) Polyclonal Antibody detects endogenous levels of Histone H2B protein only when acetylated at K5.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

18kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.

细胞定位

Nucleus. Chromosome.

信号通路

Protein_Acetylation

功能

DNA packaging,?chromatin organization,?chromatin assembly or disassembly,?nucleosome assembly,?defense response,?response to bacterium,?chromatin assembly,?cellular macromolecular complex subunit organization,?cellular macromolecular complex assembly,?nucleosome organization,?defense response to bacterium,?macromolecular complex subunit organization,?chromosome organization,?macromolecular complex assembly,?protein-DNA complex assembly,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using Histone H2B （Acetyl-Lys5） Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B （Acetyl-Lys5） Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Acetyl-Histone H2B （K5） Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Western Blot analysis of 3T3 cells using Acetyl-Histone H2B （K5） Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000