产品名称
Acetyl Histone H2A (K5) Rabbit Polyclonal Antibody
别名
H2AFZ; H2AZ; Histone H2A.Z; H2A/z
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3015
Human Swissprot No.
P0C0S5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P0C0S5/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=51788
Mouse Swissprot No.
P0C0S6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P0C0S6
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=58940
Rat Swissprot Link
http://www.uniprot.org/uniprot/P0C0S7
免疫原
The antiserum was produced against synthesized peptide derived from human Histone H2A around the acetylated site of Lys5. AA range:1-50
特异性
Acetyl-Histone H2A (K5) Polyclonal Antibody detects endogenous levels of Histone H2A protein only when acetylated at K5.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008],
组织表达
Brain,Epithelium,Skeletal muscle,Uterus,
细胞定位
Nucleus. Chromosome.
功能
function:Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.,mass spectrometry:Monoisotopic, not modified PubMed:16457589,PTM:Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.,PTM:Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.,PTM:Not phosphorylated.,similarity:Belongs to the histone H2A family.,subunit:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
