产品名称
Acetyl Histone H1 (K25) Rabbit Polyclonal Antibody
别名
H1FOO; H1OO; OSH1; Histone H1oo; Oocyte-specific histone H1; Oocyte-specific linker histone H1; osH1;H1K25AC
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=132243
Human Swissprot No.
Q8IZA3
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8IZA3/entry
Mouse Swissprot No.
Q8VIK3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8VIK3
免疫原
The antiserum was produced against synthesized peptide derived from human Histone H1 around the acetylated site of Lys25. AA range:131-180
特异性
Acetyl-Histone H1 (K25) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when acetylated at K25.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Oct 2015],
细胞定位
Cytoplasm . Nucleus . Chromosome .
功能
function:May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.,similarity:Belongs to the histone H1/H5 family.,tissue specificity:Oocyte-specific.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
