BD-PT3174-NOS3 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NOS3 Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT3174

产品名称

NOS3 Rabbit Polyclonal Antibody

别名

NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

类别

常规抗体

基因名称

NOS3

蛋白名称

Nitric oxide synthase endothelial

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4846

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4846

Human Swissprot No.

P29474

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P29474/entry

Mouse Gene ID

18127

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18127

Mouse Swissprot No.

P70313

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P70313

Rat Gene ID

24600

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24600

Rat Swissprot No.

Q62600

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q62600

免疫原

The antiserum was produced against synthesized peptide derived from human eNOS. AA range:1145-1194

特异性

NOS3 Polyclonal Antibody detects endogenous levels of NOS3 protein.

稀释度

WB 1:500-2000, IF 1:50-300, IHC 1:50-300

预测分子量

130-140kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

组织表达

Platelets, placenta, liver and kidney.

细胞定位

Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

信号通路

Regulates Angiogenesis; AMPK; PI3K/Akt; Protein_Acetylation

功能

catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subcellular location:Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.,subunit:Homodimer. Interacts with NOSIP and NOSTRIN.,tissue specificity:Platelets, placenta, liver and kidney.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using eNOS Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549, K562, and COLO205 cells, using eNOS Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using eNOS antibody.

Immunofluorescence analysis of rat-lung tissue. 1,NOS3 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-kidney tissue. 1,NOS3 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,NOS3 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of A549 cells using NOS3 Polyclonal Antibody diluted at 1：1000

Western blot analysis of mouse-brain lysis using NOS3 antibody. Antibody was diluted at 1:1000