BD-PT3169-NOS2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NOS2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT3169

产品名称

NOS2 Rabbit Polyclonal Antibody

别名

NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

类别

常规抗体

基因名称

NOS2, INOS

蛋白名称

Nitric oxide synthase inducible

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4843

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4843

Human Swissprot No.

P35228

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P35228/entry

Mouse Gene ID

18126

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18126

Mouse Swissprot No.

P29477

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P29477

Rat Gene ID

24599

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24599

Rat Swissprot No.

Q06518

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q06518

免疫原

The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166

特异性

NOS2 Polyclonal Antibody detects endogenous levels of NOS2 protein.

稀释度

IF 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

预测分子量

131kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008],

组织表达

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes (PubMed:7504305).

细胞定位

Cytoplasm, cytosol . Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. .

信号通路

Arginine and proline metabolism;Calcium;Pathways in cancer;Small cell lung cancer;

功能

catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.,function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.,induction:By endotoxins and cytokines.,online information:Nitric oxide synthase entry,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subunit:Homodimer. Binds SLC9A3R1.,tissue specificity:Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from NIH/3T3 cells, using iNOS Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-kidney tissue. 1,NOS2 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-lung tissue. 1,NOS2 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-kidney tissue. 1,NOS2 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,NOS2 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using NOS2 Polyclonal Antibody diluted at 1：500

Western Blot analysis of SH-SY5Y cells using NOS2 Polyclonal Antibody diluted at 1：500

Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500