产品名称
Nopp140 Rabbit Polyclonal Antibody
别名
NOLC1; KIAA0035; NS5ATP13; Nucleolar and coiled-body phosphoprotein 1; 140 kDa nucleolar phosphoprotein; Nopp140; Hepatitis C virus NS5A-transactivated protein 13; HCV NS5A-transactivated protein 13; Nucleolar 130 kDa protein; Nucleolar pho
蛋白名称
Nucleolar and coiled-body phosphoprotein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9221
Human Swissprot No.
Q14978
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q14978/entry
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64896
Rat Swissprot Link
http://www.uniprot.org/uniprot/P41777
免疫原
Synthesized peptide derived from Nopp140 . at AA range: 620-700
特异性
Nopp140 Polyclonal Antibody detects endogenous levels of Nopp140 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
74,130KD(Nucleolar phosphoprotein p130)
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.,PTM:Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. There is evidence suggesting that CDC2 kinase phosphorylates p130 at the M-phase.,similarity:Contains 1 LisH domain.,subcellular location:Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.,subunit:Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II.,
组织表达
Bone marrow,Epithelium,Leukemia,Liver,Lung,Placenta,T-cell,
细胞定位
Nucleus, nucleolus . Cytoplasm . Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase. .
功能
function:Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.,PTM:Undergoes rapid and massive phosphorylation/dephosphorylation cycles on CK2 and PKC sites. There is evidence suggesting that CDC2 kinase phosphorylates p130 at the M-phase.,similarity:Contains 1 LisH domain.,subcellular location:Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase.,subunit:Interacts with RNA polymerase I 194 kDa subunit (RPA194) and with casein kinase-II.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
