BD-PT3165-Nop132 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Nop132 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT3165

产品名称

Nop132 Rabbit Polyclonal Antibody

别名

NOL8; C9orf34; NOP132; Nucleolar protein 8; Nucleolar protein Nop132

类别

常规抗体

基因名称

NOL8

蛋白名称

Nucleolar protein 8

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

55035

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55035

Human Swissprot No.

Q76FK4

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q76FK4/entry

Mouse Swissprot No.

Q3UHX0

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q3UHX0

免疫原

The antiserum was produced against synthesized peptide derived from human NOL8. AA range:1118-1167

特异性

Nop132 Polyclonal Antibody detects endogenous levels of Nop132 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

130kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth (Sekiguchi et al., 2004 [PubMed 14660641]).[supplied by OMIM, Mar 2008],

组织表达

Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.

细胞定位

Nucleus, nucleolus . Localizes in the nucleolar-organizing region during ribosome biogenesis.

功能

function:Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.,induction:Up-regulated in diffuse-type gastric cancers.,PTM:Phosphorylated.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7.,tissue specificity:Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of NOL8 Antibody. The lane on the right is blocked with the NOL8 peptide.

Immunohistochemistryt analysis of paraffin-embedded human brain, using NOL8 Antibody. The lane on the right is blocked with the NOL8 peptide.

Western blot analysis of the lysates from HepG2 cells using NOL8 antibody.

Western Blot analysis of 3T3 cells using Nop132 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of NIH-3T3 cells using Nop132 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.