产品名称
NIFK Rabbit Polyclonal Antibody
别名
MKI67IP; NIFK; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK
蛋白名称
MKI67 FHA domain-interacting nucleolar phosphoprotein
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84365
Human Swissprot No.
Q9BYG3
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9BYG3/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=67949
Mouse Swissprot No.
Q91VE6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q91VE6
免疫原
The antiserum was produced against synthesized peptide derived from human NIFK. AA range:200-249
特异性
NIFK Polyclonal Antibody detects endogenous levels of NIFK protein.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009],
组织表达
Brain,Cervix carcinoma,Epithelium,Lung,Plac
细胞定位
Nucleus, nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67.
功能
PTM:Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Localizes to mitotic chromosomes in conjunction with MKI67.,subunit:Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
