产品名称
NF-M Rabbit Polyclonal Antibody
别名
NEFM; NEF3; NFM; Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
蛋白名称
Neurofilament medium polypeptide
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4741
Human Swissprot No.
P07197
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P07197/entry
Mouse Swissprot No.
P08553
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P08553
免疫原
The antiserum was produced against synthesized peptide derived from human NF-M. AA range:542-591
特异性
NF-M Polyclonal Antibody detects endogenous levels of NF-M protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
neurofilament, medium polypeptide(NEFM) Homo sapiens Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008],
组织表达
Brain,Brain cortex,Fetal brain cortex,
细胞定位
Cytoplasm, cytoskeleton . Cell projection, axon .
信号通路
Amyotrophic lateral sclerosis (ALS);
功能
function:Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.,PTM:Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.,PTM:There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.,similarity:Belongs to the intermediate filament family.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
