产品名称
NEIL1 Rabbit Polyclonal Antibody
别名
NEIL1; Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; NEH1; Nei-like protein 1
蛋白名称
Endonuclease 8-like 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=79661
Human Swissprot No.
Q96FI4
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q96FI4/entry
Mouse Swissprot No.
Q8K4Q6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K4Q6
免疫原
The antiserum was produced against synthesized peptide derived from human NEIL1. AA range:291-340
特异性
NEIL1 Polyclonal Antibody detects endogenous levels of NEIL1 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012],
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus . Chromosome . During mitosis, associates with centrosomes and condensed chromatin.
信号通路
Base excision repair;
功能
catalytic activity:Removes damaged bases from DNA, leaving an abasic site.,catalytic activity:The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.,function:Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.,induction:Up-regulated during S-phase.,similarity:Belongs to the FPG family.,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
