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NEEP21 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT3028
产品名称
NEEP21 Rabbit Polyclonal Antibody
别名
NSG1; D4S234; Neuron-specific protein family member 1; Brain neuron cytoplasmic protein 1
类别
常规抗体
基因名称
NSG1
蛋白名称
Neuron-specific protein family member 1
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
27065
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=27065
Human Swissprot No.
P42857
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P42857/entry
Mouse Gene ID
18196
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18196
Mouse Swissprot No.
Q62092
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q62092
Rat Gene ID
25247
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25247
Rat Swissprot No.
P02683
Rat Swissprot Link
http://www.uniprot.org/uniprot/P02683
免疫原
The antiserum was produced against synthesized peptide derived from human NSG1. AA range:112-161
特异性
NEEP21 Polyclonal Antibody detects endogenous levels of NEEP21 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
预测分子量
26kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
similarity:Belongs to the NSG family.,
组织表达
Amygdala,Brain,Cerebellum,Testis,
细胞定位
Membrane ; Single-pass type II membrane protein . Golgi apparatus, trans-Golgi network membrane . Endosome membrane . Cell projection, dendrite . Early endosome membrane . Late endosome membrane . Lysosome lumen . Recycling endosome membrane . Cytoplasmic vesicle membrane . Golgi apparatus, Golgi stack membrane . Endosome, multivesicular body membrane . Endoplasmic reticulum membrane . Endocytosed from the cell surface, thus enters into early endosomes, trafficks to late endosomes and degradates in lysosomes (By similarity). Endoplasmic reticulum targeting is essential for apoptosis (PubMed:20599942). Found in both stationary and motile endosomes. A previous study supports a type I membrane protein topology (By similarity). .
功能
similarity:Belongs to the NSG family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from COLO cells, using NSG1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from K562 cells using NSG1 antibody.

Western Blot analysis of various cells using NEEP21 Polyclonal Antibody diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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