产品名称
Neddylin Rabbit Polyclonal Antibody
别名
NEDD8; NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4738
Human Swissprot No.
Q15843
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15843/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18002
Mouse Swissprot No.
P29595
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P29595
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25490
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q71UE8
免疫原
The antiserum was produced against synthesized peptide derived from human NEDD8. AA range:10-59
特异性
Neddylin Polyclonal Antibody detects endogenous levels of Neddylin protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.,PTM:Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.,similarity:Belongs to the ubiquitin family.,subcellular location:Mainly nuclear.,subunit:Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.,tissue specificity:Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.,
组织表达
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
细胞定位
Nucleus . Mainly nuclear. .
功能
function:Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.,PTM:Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.,similarity:Belongs to the ubiquitin family.,subcellular location:Mainly nuclear.,subunit:Directly interacts with NUB1 and AHR. Covalently attached to cullins and p53.,tissue specificity:Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
