BD-PT2988-N-cadherin Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

N-cadherin Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT2988

产品名称

N-cadherin Rabbit Polyclonal Antibody

别名

CDH2; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD antigen CD325

类别

常规抗体

基因名称

CDH2

蛋白名称

Cadherin-2

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1000

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1000

Human Swissprot No.

P19022

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P19022/entry

Mouse Gene ID

12558

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12558

Mouse Swissprot No.

P15116

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P15116

Rat Gene ID

83501

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=83501

Rat Swissprot No.

Q9Z1Y3

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q9Z1Y3

免疫原

The antiserum was produced against synthesized peptide derived from human CDH2. AA range:721-770

特异性

N-cadherin Polyclonal Antibody detects endogenous levels of N-cadherin protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

140kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015],

组织表达

Brain,Epithelium,Liver,

细胞定位

Cell membrane ; Single-pass type I membrane protein . Cell membrane, sarcolemma . Cell junction . Cell surface . Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. .

信号通路

Cell adhesion molecules (CAMs);Arrhythmogenic right ventricular cardiomyopathy (ARVC);

功能

function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism.,similarity:Contains 5 cadherin domains.,subunit:Interacts with CDCP1.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from COLO205 and K562 cells, using CDH2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-lung tissue. 1,N-cadherin Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-kidney tissue. 1,N-cadherin Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-kidney tissue. 1,N-cadherin Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,N-cadherin Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,N-cadherin Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,N-cadherin Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using N-cadherin Polyclonal Antibody diluted at 1：1000

Western Blot analysis of K562 cells using N-cadherin Polyclonal Antibody diluted at 1：1000

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.