产品名称
NARF Rabbit Polyclonal Antibody
别名
NARF; Nuclear prelamin A recognition factor; Iron-only hydrogenase-like protein 2; IOP2
蛋白名称
Nuclear prelamin A recognition factor
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26502
Human Swissprot No.
Q9UHQ1
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9UHQ1/entry
Mouse Swissprot No.
Q9CYQ7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9CYQ7
免疫原
The antiserum was produced against synthesized peptide derived from human NARF. AA range:262-311
特异性
NARF Polyclonal Antibody detects endogenous levels of NARF protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It
组织表达
Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain.
功能
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the NARF family.,subunit:Interacts with LMNA and binds to the farnesylated C-terminal domain.,tissue specificity:Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
