产品名称
NAB2 Rabbit Polyclonal Antibody
别名
NAB2; MADER; NGFI-A-binding protein 2; EGR-1-binding protein 2; Melanoma-associated delayed early response protein; Protein MADER
蛋白名称
NGFI-A-binding protein 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4665
Human Swissprot No.
Q15742
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15742/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17937
Mouse Swissprot No.
Q61127
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61127
免疫原
The antiserum was produced against synthesized peptide derived from human NAB2. AA range:261-310
特异性
NAB2 Polyclonal Antibody detects endogenous levels of NAB2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],
组织表达
Widely expressed at low levels. Highly expressed in melanoma cell lines.
细胞定位
Nucleus . Isoform 2 is not localized to the nucleus. .
功能
domain:The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.,domain:The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.,function:Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.,induction:By serum and PMA stimulation.,similarity:Belongs to the NAB family.,subcellular location:Isoform 2 is not localized to the nucleus.,subunit:Homomultimers may associate with EGR1 bound to DNA.,tissue specificity:Widely expressed at low levels. Highly expressed in melanoma cell lines.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
