BD-PT2973-Na+/K+-ATPase α1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Na+/K+-ATPase α1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2973

产品名称

Na+/K+-ATPase α1 Rabbit Polyclonal Antibody

别名

ATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1

类别

常规抗体

基因名称

ATP1A1

蛋白名称

Sodium/potassium-transporting ATPase subunit alpha-1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

476

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=476

Human Swissprot No.

P05023

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P05023/entry

Mouse Gene ID

11928

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11928

Mouse Swissprot No.

Q8VDN2

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8VDN2

Rat Gene ID

24211

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24211

Rat Swissprot No.

P06685

Rat Swissprot Link

http://www.uniprot.org/uniprot/P06685

免疫原

The antiserum was produced against synthesized peptide derived from human ATPase. AA range:5-54

特异性

Na+/K+-ATPase α1 Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

112kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

组织表达

Brain,Cerebellum,Cervix,Placenta,Retinal pigment epithelium

细胞定位

Basolateral cell membrane ; Multi-pass membrane protein . Cell membrane, sarcolemma ; Multi-pass membrane protein . Cell projection, axon . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .

信号通路

Cardiac muscle contraction;Aldosterone-regulated sodium reabsorption;

功能

catalytic activity:ATP + H(2)O + Na(+)(In) + K(+)(Out) = ADP + phosphate + Na(+)(Out) + K(+)(In).,function:This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.,PTM:Phosphorylation on Tyr-10 modulates pumping activity.,similarity:Belongs to the cation transport ATPase (P-type) family.,similarity:Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of COS7 cells, using ATPase Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ATPase Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with PMA 125ng/ml 30‘, using ATPase Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Na+/K+-ATPase α1 Polyclonal Antibody diluted at 1：1000

Western Blot analysis of 293 cells using Na+/K+-ATPase α1 Polyclonal Antibody diluted at 1：1000

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.