BD-PT2962-N4BP1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

N4BP1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2962

产品名称

N4BP1 Rabbit Polyclonal Antibody

别名

N4BP1; KIAA0615; NEDD4-binding protein 1; N4BP1

类别

常规抗体

基因名称

N4BP1

蛋白名称

NEDD4-binding protein 1

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

9683

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9683

Human Swissprot No.

O75113

Human Swissprot Link

http://www.uniprot.org/uniprotkb/O75113/entry

Mouse Swissprot No.

Q6A037

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q6A037

免疫原

The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432

特异性

N4BP1 Polyclonal Antibody detects endogenous levels of N4BP1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

预测分子量

100kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,

组织表达

Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.

细胞定位

Cytoplasm, cytosol . Nucleus . Nucleus, nucleolus . Nucleus, PML body . Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. .

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from MCF-7 and Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of K562 cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.