产品名称
MYPT1 Rabbit Polyclonal Antibody
别名
PPP1R12A; MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit
蛋白名称
Protein phosphatase 1 regulatory subunit 12A
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4659
Human Swissprot No.
O14974
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O14974/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17931
Mouse Swissprot No.
Q9DBR7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9DBR7
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=116670
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q10728
免疫原
The antiserum was produced against synthesized peptide derived from human MYPT1. AA range:621-670
特异性
MYPT1 Polyclonal Antibody detects endogenous levels of MYPT1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph
组织表达
Expressed in striated muscles, specifically in type 2a fibers (at protein level).
细胞定位
Cytoplasm . Cytoplasm, cytoskeleton, stress fiber . Also along actomyosin filaments. .
信号通路
Vascular smooth muscle contraction;Focal adhesion;Long-term potentiation;Regulates Actin and Cytoskeleton;
功能
function:Regulates myosin phosphatase activity.,PTM:Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 6 ANK repeats.,subcellular location:Along actomyosin filaments and stress fibers.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
