BD-PT2944-Myogenin Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Myogenin Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2944

产品名称

Myogenin Rabbit Polyclonal Antibody

别名

MYOG; BHLHC3; MYF4; Myogenin; Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4

类别

常规抗体

基因名称

MYOG

蛋白名称

Myogenin

推荐应用

反应种属

Human,Mouse,Rat,Pig(Test by out customer),Pig

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4656

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4656

Human Swissprot No.

P15173

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P15173/entry

Mouse Gene ID

17928

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17928

Mouse Swissprot No.

P12979

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P12979

Rat Gene ID

29148

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29148

Rat Swissprot No.

P20428

Rat Swissprot Link

http://www.uniprot.org/uniprot/P20428

免疫原

The antiserum was produced against synthesized peptide derived from human Myogenin. AA range:50-99

特异性

Myogenin Polyclonal Antibody detects endogenous levels of Myogenin protein.

稀释度

IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200

预测分子量

39kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008],

组织表达

Eye,Skeletal muscle,

细胞定位

Nucleus. Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos. .

功能

function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from A549 cells, using Myogenin antibody.

Western Blot analysis of various cells using Myogenin Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human liver. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.