产品名称
MYLIP Rabbit Polyclonal Antibody
别名
MYLIP; BZF1; IDOL; BM-023; PP5242; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR
蛋白名称
E3 ubiquitin-protein ligase MYLIP
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29116
Human Swissprot No.
Q8WY64
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8WY64/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=218203
Mouse Swissprot No.
Q8BM54
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BM54
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=306825
Rat Swissprot Link
http://www.uniprot.org/uniprot/D3ZDI6
免疫原
The antiserum was produced against synthesized peptide derived from human MYLIP. AA range:161-210
特异性
MYLIP Polyclonal Antibody detects endogenous levels of MYLIP protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitously expressed.
细胞定位
Cytoplasm . Cell membrane ; Peripheral membrane protein .
功能
developmental stage:Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.,domain:The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.,function:E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Stable or transient overexpression of MIR induces proteasomal degradation of MRLC inhibiting neurite outgrowth in presence of NGF. MIR counteracts the stabilization of MRLC by MIR-interacting saposin-like protein (MSAP/TMEM4) and reduces MSAP-stimulated neurite outgrowth.,pathway:Protein modification; protein ubiquitination.,PTM:Autoubiquitinated.,similarity:Contains 1 FERM domain.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with myosin regulatory light chain (MRLC) and TMEM4.,tissue specificity:Ubiquitously expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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