BD-PT2928-MyD88 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MyD88 Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT2928

产品名称

MyD88 Rabbit Polyclonal Antibody

别名

MYD88; Myeloid differentiation primary response protein MyD88

类别

常规抗体

基因名称

MyD88

蛋白名称

Myeloid differentiation primary response protein MyD88

推荐应用

反应种属

Human,Mouse,Rat,Pig

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4615

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4615

Human Swissprot No.

Q99836

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q99836/entry

Mouse Gene ID

17874

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17874

Mouse Swissprot No.

P22366

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P22366

Rat Gene ID

301059

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=301059

Rat Swissprot No.

Q6Y1S1

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q6Y1S1

免疫原

The antiserum was produced against synthesized peptide derived from human MyD88. AA range:171-220

特异性

MyD88 Polyclonal Antibody detects endogenous levels of MyD88 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunocytochemistry: 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

33kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010],

组织表达

Ubiquitous.

细胞定位

Cytoplasm . Nucleus .

信号通路

Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Toll_Like;

功能

disease:Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also called recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.,function:Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. May be involved in myeloid differentiation.,similarity:Contains 1 death domain.,similarity:Contains 1 TIR domain.,subunit:Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1 and IRAK2 via their respective TIR domains. Interacts with IL1RL1.,tissue specificity:Ubiquitous.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of MyD88 antibody in paraffin-embedded human brain tissue.

Western blot analysis of lysate from COLO cells, using MyD88 antibody.

Immunofluorescence analysis of mouse-spleen tissue. 1,MyD88 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-lung tissue. 1,MyD88 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,MyD88 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using MyD88 Polyclonal Antibody diluted at 1：2000

Western Blot analysis of COLO205 cells using MyD88 Polyclonal Antibody diluted at 1：2000

Western blot analysis of mouse-liver mouse-heart mouse-lung Cell Lysate, antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000