产品名称
mTERF Rabbit Polyclonal Antibody
别名
MTERF; Transcription termination factor; mitochondrial; Mitochondrial transcription termination factor 1; mTERF
蛋白名称
Transcription termination factor mitochondrial
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7978
Human Swissprot No.
Q99551
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q99551/entry
Mouse Swissprot No.
Q8CHZ9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8CHZ9
免疫原
The antiserum was produced against synthesized peptide derived from human MTERF. AA range:267-316
特异性
mTERF Polyclonal Antibody detects endogenous levels of mTERF protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a mitochondrial transcription termination factor. This protein participates in attenuating transcription from the mitochondrial genome; this attenuation allows higher levels of expression of 16S ribosomal RNA relative to the tRNA gene downstream. The product of this gene has three leucine zipper motifs bracketed by two basic domains that are all required for DNA binding. There is evidence that, for this protein, the zippers participate in intramolecular interactions that establish the three-dimensional structure required for DNA binding. [provided by RefSeq, Jul 2008],
组织表达
Brain,Colon,Placenta,Uterus,
功能
domain:Composed of three leucine zippers, one of which is bipartite, and two widely spaced basic domains. There is evidence that the leucine zippers form and intramolecular three-stranded coiled-coil that brings the basic domains together to form a DNA-binding motif.,function:Transcription termination factor. Binds to a 28 bp region within the tRNA(Leu(uur)) gene at a position immediately adjacent to and downstream of the 16S rRNA gene, this region comprises a tridecamer sequence critical for directing accurate termination. Probably requires one or more components for termination activity.,PTM:Phosphoprotein with mostly four phosphate groups. While the DNA-binding activity is unaffected by the phosphorylation state, only the phosphorylated form of the protein is active for termination activity. Functioning seems to be regulated by phosphorylation.,similarity:Belongs to the mTERF family.,subunit:Monomer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
