BD-PT2895

MRTF-A Rabbit Polyclonal Antibody

MKL1; KIAA1438; MAL; MKL/myocardin-like protein 1; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; Myocardin-related transcription factor A; MRTF-A

常规抗体

MKL1

MKL/myocardin-like protein 1

WB

Human,Mouse

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

57591

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=57591

Q969V6

http://www.uniprot.org/uniprotkb/Q969V6/entry

223701

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=223701

Q8K4J6

http://www.uniprot.org/uniprot/Q8K4J6

The antiserum was produced against synthesized peptide derived from human MKL1. AA range:10-59

MRTF-A Polyclonal Antibody detects endogenous levels of MRTF-A protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

99kD

-20°C/1 year

Polyclonal, Rabbit,IgG

The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013],

Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain (PubMed:11344311). Expressed in peripheral blood mononuclear cells (at protein level) (PubMed:26224645).

Cytoplasm . Nucleus . Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G-actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (By similarity). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (By similarity). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (By similarity). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (PubMed:24440334). .

disease:A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.,domain:The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.,function:Transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) and has the potential to modulate SRF-target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.,similarity:Contains 1 SAP domain.,similarity:Contains 2 RPEL repeats.,subunit:Forms a ternary complex with SRF on DNA. Interacts with MKL2.,tissue specificity:Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.