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MRP-S25 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT2883
产品名称
MRP-S25 Rabbit Polyclonal Antibody
别名
MRPS25; RPMS25; 28S ribosomal protein S25; mitochondrial; MRP-S25; S25mt
类别
常规抗体
基因名称
MRPS25
蛋白名称
28S ribosomal protein S25 mitochondrial
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
64432
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64432
Human Swissprot No.
P82663
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P82663/entry
Mouse Gene ID
64658
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64658
Mouse Swissprot No.
Q9D125
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9D125
Rat Gene ID
297459
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=297459
Rat Swissprot No.
Q4QR80
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q4QR80
免疫原
The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173
特异性
MRP-S25 Polyclonal Antibody detects endogenous levels of MRP-S25 protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
预测分子量
20kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],
组织表达
Lung,Spleen,
细胞定位
Mitochondrion .
功能
similarity:Belongs to the ribosomal protein S25/L51 family.,subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from 293 cells, using MRPS25 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HepG2 cells using MRPS25 antibody.

Western Blot analysis of various cells using MRP-S25 Polyclonal Antibody diluted at 1:2000

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