BD-PT2828

MRCKβ Rabbit Polyclonal Antibody

CDC42BPB; KIAA1124; Serine/threonine-protein kinase MRCK beta; CDC42-binding protein kinase beta; CDC42BP-beta; DMPK-like beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; MRCK beta; Myotonic dystrophy protein kinase-like b

常规抗体

CDC42BPB

Serine/threonine-protein kinase MRCK beta

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

9578

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9578

Q9Y5S2

http://www.uniprot.org/uniprotkb/Q9Y5S2/entry

217866

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=217866

Q7TT50

http://www.uniprot.org/uniprot/Q7TT50

113960

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=113960

Q7TT49

http://www.uniprot.org/uniprot/Q7TT49

The antiserum was produced against synthesized peptide derived from human MRCKB. AA range:1641-1690

MRCKβ Polyclonal Antibody detects endogenous levels of MRCKβ protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

194kD

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008],

Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.

Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction . Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity). .

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.,function:May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 CNH domain.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,subcellular location:Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent.,subunit:Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42.,tissue specificity:Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.