产品名称
Mnk2 Rabbit Polyclonal Antibody
别名
MKNK2; GPRK7; MNK2; MAP kinase-interacting serine/threonine-protein kinase 2; MAP kinase signal-integrating kinase 2; MAPK signal-integrating kinase 2; Mnk2
蛋白名称
MAP kinase-interacting serine/threonine-protein kinase 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2872
Human Swissprot No.
Q9HBH9
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9HBH9/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17347
Mouse Swissprot No.
Q8CDB0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8CDB0
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=299618
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q5U2N4
免疫原
Synthesized peptide derived from the Internal region of human Mnk2.
特异性
Mnk2 Polyclonal Antibody detects endogenous levels of Mnk2 protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
MAP kinase interacting serine/threonine kinase 2(MKNK2) Homo sapiens This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex.
组织表达
Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
细胞定位
[Isoform 2]: Nucleus, PML body.; [Isoform 1]: Cytoplasm.
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Insulin_Receptor;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 1 zinc ion per subunit.,cofactor:Magnesium.,function:May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.,PTM:Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Monomer. Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2. Isoform 2 interacts with ESR2.,tissue specificity:Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
