产品名称
MMP-8 Rabbit Polyclonal Antibody
别名
MMP8; CLG1; Neutrophil collagenase; Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL
蛋白名称
Neutrophil collagenase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4317
Human Swissprot No.
P22894
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P22894/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17394
Mouse Swissprot No.
O70138
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O70138
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=63849
Rat Swissprot Link
http://www.uniprot.org/uniprot/O88766
免疫原
The antiserum was produced against synthesized peptide derived from human MMP-8. AA range:418-467
特异性
MMP-8 Polyclonal Antibody detects endogenous levels of MMP-8 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
matrix metallopeptidase 8(MMP8) Homo sapiens This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],
细胞定位
Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.
功能
catalytic activity:Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.,cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 3 calcium ions per subunit.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:Cannot be activated without removal of the activation peptide.,function:Can degrade fibrillar type I, II, and III collagens.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,subcellular location:Stored in intracellular granules.,tissue specificity:Neutrophils.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
