产品名称
MMP-19 Rabbit Polyclonal Antibody
别名
MMP19; MMP18; RASI; Matrix metalloproteinase-19; MMP-19; Matrix metalloproteinase RASI; Matrix metalloproteinase-18; MMP-18
蛋白名称
Matrix metalloproteinase-19
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4327
Human Swissprot No.
Q99542
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q99542/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=58223
Mouse Swissprot No.
Q9JHI0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JHI0
免疫原
The antiserum was produced against synthesized peptide derived from human MMP-19. AA range:11-60
特异性
MMP-19 Polyclonal Antibody detects endogenous levels of MMP-19 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded protein is secreted as an inactive proprotein, which is activated upon cleavage by extracellular proteases. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013],
组织表达
Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients (PubMed:8920941).
细胞定位
Secreted, extracellular space, extracellular matrix .
功能
alternative products:Additional isoforms seem to exist,catalytic activity:Cleaves aggrecan at the 360-Ser-|-Phe-361 site.,cofactor:Binds 1 zinc ion per subunit.,cofactor:Calcium.,disease:May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,enzyme regulation:Strongly inhibited by TIMP-2, TIMP-3 and TIMP-4, while TIMP-1 is less efficient.,function:Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.,PTM:Activated by autolytic cleavage after Lys-97.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,tissue specificity:Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
