BD-PT2796-MMP-13 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MMP-13 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT2796

产品名称

MMP-13 Rabbit Polyclonal Antibody

别名

MMP13; Collagenase 3; Matrix metalloproteinase-13; MMP-13

类别

常规抗体

基因名称

MMP13

蛋白名称

Collagenase 3

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

4322

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4322

Human Swissprot No.

P45452

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P45452/entry

Mouse Swissprot No.

P33435

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P33435

免疫原

The antiserum was produced against synthesized peptide derived from human MMP-13. AA range:10-59

特异性

MMP-13 Polyclonal Antibody detects endogenous levels of MMP-13 protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

预测分子量

55kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016],

组织表达

Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.

细胞定位

Secreted, extracellular space, extracellular matrix . Secreted .

信号通路

Angiogenesis

功能

cofactor:Binds 2 zinc ions per subunit.,cofactor:Binds 4 calcium ions per subunit.,disease:Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common.,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.,function:Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.,similarity:Belongs to the peptidase M10A family.,similarity:Contains 4 hemopexin-like domains.,tissue specificity:Seems to be specific to breast carcinomas.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HepG2 cells, using MMP-13 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MMP-13 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, using MMP-13 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using MMP-13 Polyclonal Antibody diluted at 1：500

Western blot analysis of mouse-brain lysis using MMP-13 antibody. Antibody was diluted at 1:500

Immunohistochemical analysis of paraffin-embedded Human Colon cancer. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Mammary cancer. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.