产品名称
MLF1 Rabbit Polyclonal Antibody
别名
MLF1; Myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1
蛋白名称
Myeloid leukemia factor 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4291
Human Swissprot No.
P58340
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P58340/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17349
Mouse Swissprot No.
Q9QWV4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9QWV4
免疫原
Synthesized peptide derived from MLF1 . at AA range: 130-210
特异性
MLF1 Polyclonal Antibody detects endogenous levels of MLF1 protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
myeloid leukemia factor 1(MLF1) Homo sapiens This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010],
组织表达
Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
细胞定位
Cytoplasm . Nucleus . Cell projection, cilium . Cytoplasm, cytoskeleton, cilium basal body . Shuttles between the cytoplasm and nucleus. .
功能
disease:A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.,function:Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.,PTM:Phosphorylation is required for binding to YWHAZ.,similarity:Belongs to the MLF family.,subcellular location:In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.,subunit:Interacts with MLF1IP. Also interacts with NRBP1/MADM, YWHAZ/14-3-3-zeta and HNRPUL2/MANP. NRBP1 recruits a serine kinase which phosphorylates both itself and MLF1. Phosphorylated MLF1 then binds to YWHAZ and is retained in the cytoplasm. Retained in the nucleus by binding to HNRPUL2. Binds to COPS3/CSN3 which is required for suppression of RFWD2 and activation of p53.,tissue specificity:Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
